Here are the sequences of primers and Taqman probes used in “Multiplex three dimensional brain gene expression mapping in a mouse model of Parkinson’s disease

 

Gene

Forward Primer

Taqman Probe

Reverse Primer

Ecm1a

5’-TGCCGCAGCGACACAA-3’

5’-6Fam-CCACACAAGCTTCAAACAGTCTAGGCGG-TAMRA-3’

5’-CAAAATTGGGTCATTGCATCCT-3’

Ap1b1b

5’-GGGCTGCATCCGAGTTGA-3’

5’-6Fam-TCCGGAGTGGTTCACACAAGTACTCTGTAATCTT-TAMRA-3’

5’-CACATATGGGTCTTCATCCTTCAG-3’

Psme1c

5’-TGATGACCAACCTTCACACCAA-3’

5’-6Fam-CTGGAAGGCTTCCACACGCAGATCTC-TAMRA-3’

5’-TCACCCCTCTCGGAGAAGTACTT-3’

S100a6d

5’-CCCCTCGACCACTCCTTTG-3’

5’-6Fam-CGCTTCGCTGTCGACCGTGC-TAMRA-3’

5’-TGACTGATCACTGGGCTAGAAGAA-3’

Stk2e

5’-GCAAGTTTATTGTCGATGGTGTAGA-3’

5’-6Fam-ACATCGAAGATAGTTACAGACAGCGACTCCAAAA-TAMRA-3’

5’-CCTGAGAAAGCGCAGTTCCT-3’

THf

5’-TGTTGGCTGACCGCACAT-3’

5’-6Fam-TGCCCAGTTCTCCCAGGACATTGG-TAMRA-3’

5’-AAGCCCCCAGAGATGCAAG-3’

The unigene symbols displayed in the table have the following gene descriptions. a- extracellular matrix protein 1, b-adaptor protein complex AP-1, beta 1 subunit, seven in absentia 1A, c-proteasome (prosome, macropain) 28 subunit, alpha, d-S100 calcium binding protein A6 (calcyclin), e-serine/threonine kinase 2, f-tyrosine hydroxylase.

 

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